Semi Prep HPLC Column, RP C12, 10um, 100A. 10mm ID x 50mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C12 is a unique reversed-phase semi-preparative HPLC stationary phase based on an adamantane bonded ligand, a rigid diamond-like carbon structure that provides distinctive chromatographic selectivity compared to traditional alkyl phases. The combination of high-purity silica, a narrow particle size distribution, low metal content, complete double endcapping, and the rigid bonded phase design makes this column particularly effective for purifying complex mixtures while supporting rapid equilibration between runs. The phase generally provides retention that falls between C8 and C18 columns, making it an excellent choice when C18 retention is excessive and C8 retention is insufficient.
68012-SP50 is a semi-preparative HPLC column with dimensions of 10 mm ID x 50 mm length, packed with 10 µm RP C12 particles featuring a 100 Å pore size. The 10 mm internal diameter provides significantly greater sample loading capacity than analytical columns, while the shorter 50 mm length is optimized for rapid purification cycles, high-throughput processing, and efficient method optimization. The rigid adamantane bonded phase promotes rapid re-equilibration during gradient methods, while the 10 µm particle size supports higher flow rates and lower operating backpressure. Typical operating flow rates are approximately 8–15 mL/min depending on sample load, mobile phase composition, and system capabilities.
Compatible with reversed-phase mobile phases, including 100% aqueous conditions, this column maintains reproducible retention without loss of performance between runs. The unique adamantane bonded phase can provide valuable selectivity advantages when purifying hydrophobic and moderately hydrophobic compounds from complex sample matrices. Designed for purification and fraction collection rather than routine analytical HPLC, this column is ideal for pharmaceutical research, synthetic chemistry, natural product isolation, process development, and method scale-up. The shorter column format is often selected when throughput and loading capacity are prioritized over maximum chromatographic resolution. A specialized semi-preparative HPLC system capable of higher flow rates, larger injection volumes, and fraction collection is required. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
Unique adamantane-based RP C12 stationary phase
Fully double endcapped chemistry
High-purity silica with low metal content
Narrow particle size distribution
Compatible with 100% aqueous mobile phases
Rapid equilibration between runs
Excellent for complex mixture purification
Retention between C8 and C18 phases
Increased sample loading capacity
Fast purification cycles with 50 mm column length
Typical Applications
Semi-preparative HPLC purification
Fraction collection
Compound isolation
Pharmaceutical research
Synthetic chemistry
Natural product purification
Process development
Method scale-up
Production support purification
Complex mixture purification
Specifications
Product Type: Semi-Preparative HPLC Column
Stationary Phase: RP C12
Chromatography Mode: Reversed Phase
Bonded Phase: Adamantane-Based C12 Ligand
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 100 Å
Column Dimensions: 10 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 8–15 mL/min
Brand: Cogent
Additional Resources
Special Order Item Information
Important Notes
Intended for purification and fraction collection rather than routine analytical HPLC
Compatible with 100% aqueous mobile phases
Excellent choice when C18 retention is too great and C8 retention is insufficient
Rapid re-equilibration can improve purification throughput
Increased loading capacity compared to analytical columns
Short column length supports rapid purification cycles and method optimization
Requires a semi-preparative HPLC system capable of higher flow rates and fraction collection
Special Order Item